RESUMO
Aim: The aim of the present study was to evaluate the different doses of Butyric acid [BA] and Arsenic [As] in liver mitochondria oxidative stress and pancreatic islet insulin secretion of male mouse
Background: BA is found in many foods and As as a toxic metal is present in drinking water. They can induce oxidative stress in tissues
Methods: In this experimental study, Liver mitochondria were isolated by administration of the different centrifugation method and pancreatic islets were isolated by collagenase method. Mitochondria were incubated by BA [35, 75, 150, 300 microM] and As [20, 50, 100, 200 microM] as the islets were incubated by BA [250, 500, 1000, 1500 microM] and As [50, 100, 200 microM] for 1 hour. At the end of the experiment, mitochondrial viability and membrane potential, ROS, MDA, GSH and islets insulin secretion were measured by their specific methods
Results: BA and As administration increased mitochondrial levels of ROS, MDA and decreased GSH and pancreatic islet insulin secretion in a dose dependent manner [p<0.05]. The doses of BA 75microM and As 100microM have been revealed the most mitochondria toxic concentrations. Also, the doses of 1000microM for BA and 100microM for As were considered as reducing concentrations for islets insulin secretion. Additionally, co administration of them intensified more these effects
Conclusion: Alone or in combination administration of BA and As induced oxidative stress in liver mitochondria and decreased insulin secretion of pancreatic islets
RESUMO
To evaluate the protective effect of cetrizine against bleomycin induced pulmonary fibrosis in rats. Male Sprague-Dawley rats [n=30], received an intratracheal injection of bleomycin [7.5 IU/kg] in saline solution for induction of pulmonary fibrosis. Two treatment groups received daily cetirizine five and 20mg/kg/day, seven days before and four weeks after administering a single-dose bleomycin [7.5IU/kg]. The cytokines [IL-8, TNF-alpha, TGF-alpha1] through ELISA kits, the amount of collagen in the lungs [hydroxyproline content], and pharmacological activity of the lung strip tissues were determined. The cytokine levels have been decreased in the treated groups by cetirizine 5 [p< 0.05] and 20 [p<0.01] mg/kg/day, in comparison to positive control group. Cetirizine may have a protective effect against bleomycine induced pulmonary fibrosis as evident by the reduction of the severity of lung tissue changes, collagen amounts and cytokines levels caused by bleomycine in rats lungs tissues